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Cloning #3
Project: Surface Display
Date: Thu, 25 Aug 2011 18:25:40 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 18:30:49 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
Ligation:
INP 50uL rxn
- vector (4.8 ug/mL): 20 uL
- INP (17.6 ug/mL): 22 uL
- ligase: 1 uL
- ligase buffer: 5 uL
- H2O: 2 uL
Negative control 30 uL rxn
- vector: 6 uL
- H2O: 20 uL
- ligase: 1 uL
- ligase buffer: 3 uL
Incubate at 16 degrees for 1 hr.
Transformation: see protocal 7/13/11
Single digestion of 8/24/11 clones
- 1 uL BamHI
- 3 buffer 4
- 26 uL cloned plasmid DNA
Incubate at 37 degrees overnight
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