Team:Minnesota/dingx055-07122011161717-157

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Digestion of INP

Project: Surface Display

Date: Tue, 12 Jul 2011 16:17:17 GMT

Author: Viki Ding (dingx055)

Access: Public

Revision History:

Mon, 26 Sep 2011 16:20:00 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)

Objective: double digestion, prepare for ligation

30 uL reaction

INP tube

1 uL BamHI

1 uL XhoI

3 uL buffer 4

0.3 uL BSA

24.7 uL INP DNA

pMCS5BB tube

1 uL BamHI

1 uL XhoI

3 uL buffer 4

0.3 uL BSA

24.7 uL plasmid DNA

Incubate at 37 degrees overnight.


240px