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Electroporation transformation
Project: Surface Display
Date: Sun, 12 Jun 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Tue, 23 Aug 2011 12:07:53 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
To try another method of transformation using electrocompetent cells instead of the chemical competent cells.
Procedures:
-Thaw competent cells in ice, also place cuvettes in ice.
-Add 1 uL of ligation into the thawed competent cells
-Incubate in ice for 5 minutes.
-Shock with electric current
-Add SOC or LB liquid to cuvette
-Plate
Results:
Negative= 0 colonies
OmpA Ligation= 0 colonies.
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