Team:Calgary/Notebook/Protocols
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Protocols
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Hello! Our wiki is currently under heavy construction, so please check regularly for updates. Thank you for your patience.
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Plasmid Extraction
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD Construct
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the LacI-LacZ I732901 gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
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