Team:Arizona State/Lab/Protocols/PCR

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For 50 ul reactions, prepare master mix as follows for n tubes:
10*n uL 5x Phusion buffer
4*n uL 2.5 mM dNTPs
2.5*n uL forward primer
2.5*n uL reverse primer
29.5*n uL PCR water
Add 48 uL master mix to each of seven tubes.
Label tubes blank, 0, 1, 2, 4, 8, 10
Add DNA and MgCl2 to tubes according to chart:

	blank	0	1	2	4	8	10
MgCl2	0	0	.5	1	2	4	5
DNA	0	1	1	1	1	1	1

Add 0.5 uL Phusion polymerase to each tube.
Place tubes in thermocycler.
Adjust settings as needed.

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