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Notebook - June 2011

June 29, 2011

We did a lot of work today like: 1. The miniprep of the strains containing the BioBrick rh1AB. 2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA. 3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. 4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml). 5. We did all the necessary calculations for the antibiotics concentrations.

                                 Rh1AB + psB1C3 Ectroforesis
                                      File:E29-7-11.jpg