Our control for this lab was the EBY100 yeast strain of S. cerevisiae. Cultures of EBY100 (some transformed with GFP) were grown in liquid cultures of S Raffinose media and S Raffinose/1% Galactose overnight and examined the next day on the microscope. From our observations under the microscope, EBY100 rarely aggregate into clusters, while occasionally forming clumps of several cells.
Cadherin
Since cadherins only form dimers under the presence of calcium ions, we were able to use that variable as a second control mechanism. We observed that EBY100 transformed with cadherin would not form clumps until calcium was added to the cultures. However, when EDTA was added to the cultures, the clusters seen previously had dispersed.
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