How Our System Works
All of the parts that we created this summer are designed to be fusion proteins with Aga2, a yeast protein involved in mating. Aga2 disulfide bonds with Aga1, a membrane embedded protein, such that anything fused to Aga2 is displayed on the surface of the cell.
Interaction of Aga1 with Aga2-fused protein of interest leads to Yeast Cell Surface Display
By displaying a protein that would have strong interactions with itself (or another protein), we wanted to be able to control whether or not two cells would adhere or interact with one another. An example of how two proteins would lead to cell-cell interactions is shown below:
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