Team:Northwestern/Notebook/Protocols

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RETURN TO IGEM 2010

PROJECT

RESULTS

CONSIDERATIONS

ABOUT US

NOTEBOOK

ATTRIBUTIONS

File:Protocols img1.gif

Cloning

|| Transformation || Overnight || Miniprep || 3A Assembly ||
|| PCR Amplification || PCR Purification ||

File:Protocols img22.jpg

Primers and Design

|| Primer Design || Oligo Annealing || DNA Sequencing ||
|| Linearized Plasmid Backbones ||

Protocols img3 3.jpg

Cells and Solutions

|| Top10 Chemically Competent Cells || CCMB80 Buffer ||
|| Media || Glycerol Stocks || Agarose Gels || Plates ||
|| Antibiotic Stocks ||

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