Team:EPF-Lausanne/Todo
From 2011.igem.org
Todo
Contents |
Microfluidic Chips
- Continue alignment training
- Run experiments on "chemostat" chip to check design
- Adapt design of "chemostat" chip for e-coli
Preparing the parts
-
Sequence the lysis cassette -
Double-check lysis cassette sequence
All the parts are verified, we can now assemble them!
Assembly
-
Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? - Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
Protocols
-
Describe cell cultures in miniprep protocol - Write a new protocol!
- Upload "chemostat" protocols
General
- Write-up team presentation
- Upload our initial research about transcription factors
Clean room
- Order lab notebook
-
Order storage box
Calendar