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Team:Washington/Magnetosomes/Parts
From 2011.igem.org
iGEM toolkits: submitted a total twenty-two parts to the registry:
- 10 magnetosome gene groups from mamAB operon
- 3 superassemblies of mamAB operon from set of 10
- 2 sfGFP-mam gene fusions
- 5 pGA (Gibson Assembly) vectors
Magnetosome Toolkit Favorite Parts
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590015 BBa_K590015 sfGFP_mamK_pGA1C3]
This part consists of mamK gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590016 BBa_K590016 sfGFP_mamI_pGA1C3]
This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. MamI is a membrane-localized protein that localizes magnetic vesicles to the surface of cells, thus forming characteristic magnetosome chains.
The Gibson Assembly Toolkit
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590010 BBa_K590010 pGA1A3_pLacGFP]
This is a high copy plasmid backbone which has ampicillin resistance, with pLac promoter and GFP.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590011 BBa_K590011 pGA1C3_pLacGFP]
This is a high copy plasmid backbone which has chloramphenicol resistance, with pLac promoter and GFP.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590012 BBa_K590012 pGA4C5_pLacGFP]
This is a low copy plasmid backbone which has chloramphenicol resistance, with pLac promoter and GFP.
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590013 BBa_K590013 pGA4A5_pLacGFP]
This is a low copy plasmid backbone which has ampicillin resistance, with pLac promoter and GFP .
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590014 BBa_K590014 pGA3K3_pLacGFP]
This is a medium copy plasmid backbone which has kanamycin resistance, with pLac promoter and GFP.
| Gene | AMB Number | Cluster Membership | Member of 28 genes list? (specific*/related**) | Function Summary (Vesicle chain formation, and/or biomineralization) | Gene Function |
|---|---|---|---|---|---|
| mamH | amb0961 | mamAB | Related | ||
| mamI | amb0962 | mamAB | Specific | Vesicle, (Chain Formation?) | >berkeley 2010: Loss causes no membrane formation, is localized onto chains |
| mamE | amb0963 | mamAB; mam Islet | Related | >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins | |
| mamJ | amb0964 | mamAB; mam Islet | Specific | Chain Formation | >Proper magnetosome chain organization/assembly |
| mamK | amb0965 | mamAB; mam Islet | Related | Chain Formation | >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI |
| mamL | amb0966 | mamAB; mam Islet | Specific | Vesicle, biomineralization | >berkely 2010: Crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines |
| mamM | amb0967 | mamAB | Related | >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome | |
| mamN | amb0968 | mamAB | Related | >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome | |
| mamO | amb0969 | mamAB | Related | >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome | |
| mamP | amb0970 | mamAB | Related | Biomineralization | >berkeley 2010: loss causes weak magnetic response, with large but fewer crystals |
| mamA | amb0971 | mamAB | Related | >Required for magnetosome activation; activation of vessicles | |
| mamQ | amb0972 | mamAB; mam Islet | Related | >ORF; formation/maintenance of magnetosome membranes | |
| mamR | amb0973 | mamAB | Specific | Chain formation, Biomineralization | >ORF; plays a role in controlling both particle number and size of magnetite cyrstals |
| mamB | amb0974 | mamAB | Related | Vesicle, Biomineralization | >indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation |
| mamS | amb0975 | mamAB | Specific | ||
| mamT | amb0976 | mamAB | Specific | Biomineralization | >magnetite crystal growth; participates in different steps during magnetite synthesis |
| mamU | amb0977 | mamAB | Related | ||
| mamV | amb0978 | mamAB | N/A |
