Team:Washington/Magnetosomes/Background

From 2011.igem.org

iGEM Toolkits

iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the cloning efficiency and two "toolkits" were brought to the Register as a result.

(icon of a "toolkit" )

Gibson Vector Toolkit

As a continuation of 2010 UW IGEM project, we created and submitted several plasmid backbones that are Gibson Cloning Friendly a.k.a pGA vectors.

They are pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5

Magnetosome Toolkit

] ...What’s in the Magnetosome Toolkit… -A set of 18 genes for the biominieralization of magnetic crystals -Our favorite genes in pGA vectors