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Team:Valencia/Notebook/Week4
From 2011.igem.org
WEEK 4
Engineering: We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH between different flasks.
Finally we found a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!!
pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.
Colicin production: We found that antimicrobial peptides such as defensines bring difficulties. We decide it would be better to opt for bacteriocins, easier to manage and control. We begin to document about them.
pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.
Supervision of the evolution of pH and cellular concentration in our cultures, who started working well, increasing the number of cells / ml and the absorbance data was taken at 440 nm.
Colicin production: We found that antimicrobial peptides such as defensines bring difficulties. We decide it would be better to opt for bacteriocins, easier to manage and control. We begin to document about them.
