• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

Data

team, please check this page: [1]

Favourite Parts

These are officially our three favourite parts:

[http://partsregistry.org/Part:BBa_K613015 BBa_K613015]

[http://partsregistry.org/Part:BBa_K613016 BBa_K613016]

[http://partsregistry.org/Part:BBa_K613017 BBa_K613017]

Other New parts

T7 Promoter

Our lysis selection device uses what we refer to as the wild-type T7 Promoter:

[http://partsregistry.org/Part:BBa_K613001 K613001]

In addition, we submitted the following mutants:

Variants without additional lac operator (constitutive):

[http://partsregistry.org/Part:BBa_K613002 K613002]

[http://partsregistry.org/Part:BBa_K613003 K613003]

[http://partsregistry.org/Part:BBa_K613004 K613004]

[http://partsregistry.org/Part:BBa_K613005 K613005]

[http://partsregistry.org/Part:BBa_K613006 K613006]

Variants with additional lac operator (LacI repressed):

[http://partsregistry.org/Part:BBa_K613007 K613007]

[http://partsregistry.org/Part:BBa_K613008 K613008]

[http://partsregistry.org/Part:BBa_K613009 K613009]

[http://partsregistry.org/Part:BBa_K613010 K613010]

[http://partsregistry.org/Part:BBa_K613011 K613011]

[http://partsregistry.org/Part:BBa_K613012 K613012]

TetR mutants

In-vivo testing was used to characterise these:

• V36F mutant: [http://partsregistry.org/Part:BBa_K613013 K613013]
• V36F W43S double mutant: [http://partsregistry.org/Part:BBa_K613014 K613014]
• E37A W43S T141A triple mutant: [http://partsregistry.org/Part:BBa_K613015 K613015]
• P39K mutant: [http://partsregistry.org/Part:BBa_K613016 K613016]
• Y42F K108E double mutant: [http://partsregistry.org/Part:BBa_K613018 K613018]
• P39Q Y42M double mutant: [http://partsregistry.org/Part:BBa_K613019 K613019]

Medium-strength Plac

[http://partsregistry.org/Part:BBa_K613000 K613000]

T7 promoter variants

Pre-existing parts we improved

- [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - The Berkeley Lysis Device, BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.