Team:Paris Bettencourt/Experiments/Methodologies/K090403J
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Integration
After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001). After, we want to clone them into the integrative plasmid.