Team:Freiburg/Notebook/4 September

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This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

transformation

Investigators:Theo

competent E.coli were transformed with 5mikroiter Ligation reaction from 3 September

blue light receptor

picking clones

Investigators:Sophie
No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones

Lysis cassette

3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017

Investigators:Theo
Another miniprep of the parts was done

  • S66 = modified Lysis genes K124017 in pSB1A2
  • GFP = E0040 in pSB1A2
  • S48 = Quickchange-modified K098995 in pSB1A2
  • S39 = Promotor (J23104) + RBS (B0034) in pSB1C3


As we thought, there is no band for S66, although there is DNA isolated from the mini-prep. It is probably a matter of making a stock from the false mini-prep. More answers on Monday or Tuesday



9 6 2011 Minis von S66 GFP S48 S39.Jpg

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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