Team:Groningen/project notebook/12 September 2011

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March
MTWTFSS
  [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]
April
MTWTFSS
        [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_April_2011 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_April_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_April_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_April_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]
May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1]
[http://2011.igem.org/Team:Groningen/project_notebook/2_May_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_May_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_May_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_May_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_May_2011 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8]
[http://2011.igem.org/Team:Groningen/project_notebook/9_May_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_May_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_May_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_May_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_May_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15]
[http://2011.igem.org/Team:Groningen/project_notebook/16_May_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_May_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_May_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_May_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_May_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22]
[http://2011.igem.org/Team:Groningen/project_notebook/23_May_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_May_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_May_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_May_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29]
[http://2011.igem.org/Team:Groningen/project_notebook/30_May_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_May_2011 31]
June
MTWTFSS
    [http://2011.igem.org/Team:Groningen/project_notebook/1_June_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_June_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5]
[http://2011.igem.org/Team:Groningen/project_notebook/6_June_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_June_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_June_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_June_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_June_2011 12]
[http://2011.igem.org/Team:Groningen/project_notebook/13_June_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_June_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_June_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_June_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19]
[http://2011.igem.org/Team:Groningen/project_notebook/20_June_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_June_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_June_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_June_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_June_2011 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26]
[http://2011.igem.org/Team:Groningen/project_notebook/27_June_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_June_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_June_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_June_2011 30]
July
MTWTFSS
        [http://2011.igem.org/Team:Groningen/project_notebook/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]
[http://2011.igem.org/Team:Groningen/project_notebook/4_July_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_July_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_July_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_July_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10]
[http://2011.igem.org/Team:Groningen/project_notebook/11_July_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_July_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_July_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_July_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_July_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_July_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_July_2011 17]
[http://2011.igem.org/Team:Groningen/project_notebook/18_July_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_July_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_July_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_July_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24]
[http://2011.igem.org/Team:Groningen/project_notebook/25_July_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_July_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_July_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_July_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_July_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/Team:Groningen/project_notebook/1_August_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_August_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_August_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_August_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_August_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_August_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_August_2011 7]
[http://2011.igem.org/Team:Groningen/project_notebook/8_August_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_August_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_August_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_August_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_August_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_August_2011 14]
[http://2011.igem.org/Team:Groningen/project_notebook/15_August_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_August_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_August_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_August_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_August_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_August_2011 21]
[http://2011.igem.org/Team:Groningen/project_notebook/22_August_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_August_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_August_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_August_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_August_2011 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28]
[http://2011.igem.org/Team:Groningen/project_notebook/29_August_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_August_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:Groningen/project_notebook/1_September_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_September_2011 4]
[http://2011.igem.org/Team:Groningen/project_notebook/5_September_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_September_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_September_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_September_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_September_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_September_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11]
[http://2011.igem.org/Team:Groningen/project_notebook/12_September_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_September_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_September_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_September_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_September_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_September_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_September_2011 18]
[http://2011.igem.org/Team:Groningen/project_notebook/19_September_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_September_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_September_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_September_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_October_2011 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16]
[http://2011.igem.org/Team:Groningen/project_notebook/17_October_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_October_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_October_2011 23]
[http://2011.igem.org/Team:Groningen/project_notebook/24_October_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_October_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_October_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_October_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_October_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

Jori
Parser + Blob storage


Jakub
Week 37:

  • co-transformation of autoinducing loops and promoters for measurements
  • Prepared M9 medium


Joyce

Working on the presentation for Human practices. Made a few calls.

Discussing what to do this week: I am in charge of building the constructs in the chloramphenicol vector, so we can send our
new parts to the parts registry.
This means that I have to clone approximately 30 samples

First a PCR of the plasmid backbone pSB1C3 at 13:45 afterwards: clean up and use 50ng for digestion
Per sample: (2 samples with annealing temperatures of 61,63 and 65 degrees.

Pfu 10× buffer: 5μl
dNTPs 10mM: 1μl
Forward primer: 1μl
Reverse primer: 1μl
pfu DNA polymerase: 1μl
Template: 0.5μl
MQ water: 40.5μl

PCR conditions:
Preheated lid: 111°C
Denaturation: 95°C for 3 min.
Cycle 32×:
denaturation: 95°C for 30s.
annealing: 61,63 and 65°C for 30s.
Extension: 72°C for 4 min.
Final extension: 72°C for 10 min.
Store infinite at 4°C
Finished at 17h, put samples on gel and purify the PCR products.

Making scheme for digestion and ligation of the 31 samples:

Digestion:
Digestion will be done in one reaction (so insert and vector will be in one tube, so don't use fastAP!)
Scheme:
1μl pSB1C3 (50 ng)
6μl plasmid sample
1μl EcoRI
1μl PstI
2μl FD buffer
9μl MQ water

incubate samples for 1h at 37 degrees

Clean up of the digested samples with the High Pure PCR Purification Kit
NOTE: elute in 17 μl MQ water! So it can be used for ligation immediately

Ligation:
17μl digested purified sample
1μl T4 DNA ligase
2μl T4 DNA ligase buffer

Ligate overnight at 4 °C

Purification of the plasmid backbone PCR product after finished PCR at 17:15.
Analysed at 1% agarose gel:
Digestion and ligation was not done, since the PCR of the plasmid backbone failed.
New PCR was done overnight at 19h:

Pfu 10× buffer: 5μl
dNTPs 10mM: 1μl
Forward primer: 1μl
Reverse primer: 1μl
pfu DNA polymerase: 1μl
Template: 0.5μl*
MQ water: 40.5μl
* Template DNA was diluted 1:100

PCR conditions:
Preheated lid: 111°C
Denaturation: 95°C for 3 min.
Cycle 33×:
denaturation: 95°C for 1min.
annealing: 55,60 and 65°C for 30s.
Extension: 72°C for 6 min.
Final extension: 72°C for 10 min.
Store infinite at 4°C