Team:Groningen/project characterisation mu lasr

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LasR Autoinducing Loops

Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.

Leakage of the LasR autoinducing loops

To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying the following constructs:

no-tag DAS LVA
RBS 0,07 Legenda.png
RBS 0,3
RBS 0,6
RBS 1
Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL.