LasR Autoinducing Loops
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
Leakage of the LasR autoinducing loops
To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying the following constructs:
| | no-tag | DAS | LVA |
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| RBS 0,07 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027]
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LasR1.png
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023]
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| RBS 0,3 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]
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| RBS 0,6 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607029 BBa_K607029]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607025 BBa_K607025]
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| RBS 1 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607034 BBa_K607034]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607030 BBa_K607030]
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607026 BBa_K607026]
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| Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL.
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