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Team:Valencia/Notebook/Week4
From 2011.igem.org
WEEK 4
Hardware: We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH.
Finally we find a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!!
pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.
Colicin production:
pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.
Supervision of the evolution of pH and cellular concentration in our cultures, who started working well, increasing the number of cells / ml and the absorbance data was taken at 440 nm.
Colicin production:
