• Introduction
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  • Results Summary
  • Selection System
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Lysis Selection System

One major challenge in designing new regulatory parts is to determine which combinations of transcription factors and binding sequences match. From previous research and our own MITOMI experiments, we know which DNA sequences TetR binds to, and which residues of tetR participate in binding, but we do not know how changing these residues will affect either binding affinity or specificity. Molecular dynamics simulations and other theoretical approaches have not answered those questions either. In short, we know too little about protein-DNA interaction to intelligently design transcription factors. To make up for this, we present an experimental system to select valid binding pairs from many random tetR and pTet mutants, based on an inducible lysis gene.