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Lysis Selection System

T7 Promoter Variants

Introduction

We made two families of T7 promoter variants. Each family has six designed mutants and three randomer mutants, and the difference between the two families is the addition of a lac operator sequence downstream of the T7 promoter. These promoter variants were characterized using fluorescence, revealing a wide range of promoter efficiencies.

List and Sequences of Variants

Characterization of Designed Variants

Characterization of Randomer Variants