Memory Units
cI Autoinducing Loops
The autoinducing loop driven by cI will function as our first memory unit.
We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.
| cI autoinducing loop 1 (BBa_K00) after 4 hours of incubation
|
|
|
| Number
| % of total
| Mean fluorescence
|
| Visible
| 50000
| 100
| ---
|
| Fluorescent | 9180 | 18,36 | 11419
|
| Non-Fluorescent | 34424 | 68 | 5974
|
|
| Two populations are visible blabla
|
| cI autoinducing loop 6 (BBa_K00) after 4 hours of incubation
|
|
|
| Number
| % of total
| Mean fluorescence
|
| Visible
| 50000
| 100
| ---
|
| Fluorescent | 9180 | 18,36 | 11419
|
| Non-Fluorescent | 34424 | 68 | 5974
|
|
| Only one population is visible blabla
|
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