We have added a new section for the "Plasmid backbones for advanced users" of the Parts' Registry: Genome Integration plasmids, where you could find all the plasmids of the miniTn7 BioBrick tool kit. Enjoy!

Data for our characterized new parts

1. pUC18Sfi-miniTn7BB-Gm plasmid (BBa_K510000): this plasmid harbor the basic miniTn7 which may be used for integration of BioBricks in single copy into the genomes of non-enteric bacteria, in which it is non-replicative, at the conserved attTn7 site.

2. pUC18R6KT-miniTn7BB-Gm plasmid (BBa_K510012): this plasmid may be used for transposition of BioBricks into the genome of any bacterium without the pir gen thanks to is R6K replication origin.

3. attTn7 or Tn7 insertion site (BBa_K510022): recognition and insertion site of the Tn7 transposon and its synthetic derivatives.

We have also submitted these new parts:

1. pUC18SfiI-miniTn7BB-Cm (BBa_K510001): this construct is the result of replacing the gentamycin resistance cassette in pUC18SfiI-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification.

2. pUC18SfiI-miniTn7BB-Km (BBa_K510002): replace of the gentamycin resistance cassette of pUC18Sfi-miniTn7-Gm by a kanamycin resistance cassette amplified from pSB4K5.

3. pUC18R6KT-miniTn7BB-Cm (BBa_K510013): replace of the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a chloramphenicol resistance cassette obtained from pBS1C3 by PCR amplification with these primers

4. pUC18R6KT-miniTn7BB-Km (BBa_K510014): replace of the gentamycin resistance cassette in pUC18R6KT-miniTn7BB-Gm by a kanamycin resistance cassette obtained from pBS4K5.

5. pUC18R6KT-miniTn7BB-Gm-RBS+RFP (BBa_K510015): This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.

6. pUC18R6KT-miniTn7BB-Gm-pBad/araC (BBa_K510016): This plasmid can be used as inducible expression vector in single copy by integrating the device in the genome of the working organism.

7. pUC18R6KT-miniTn7BB-Gm-tetR (BBa_K510017): This plasmid can be used as inducible expression system in single copy by integrating the device in the genome of the working organism.

8. pUC18R6KT-miniTn7BB-Gm-lacZ+GFP (BBa_K510018): This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.

9. pUC18R6KT-miniTn7BB-Gm-RFP (BBa_K510020): This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.

10. pUC18R6KT-miniTn7BB-Gm-Lux (BBa_K510021): This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism.