"
Team:EPF-Lausanne/Playground
From 2011.igem.org
Playground
Use this page to test stuff.
</html>
== In vitro TF characterization ==
We used a microfluidic based approach for characterizing TF mutants in vitro. The MITOMI method allows us to measure absolute binding affinities and specificities of transcription factors (Cite the science paper). We determined the precise binding energy landscape of the wild type TetR transcription factor. We also generated several TetR transcription factor mutants and determined the specificities of a number of the new variants.
== In vitro TF characterization ==
We used a microfluidic based approach for characterizing TF mutants in vitro. The MITOMI method allows us to measure absolute binding affinities and specificities of transcription factors (Cite the science paper). We determined the precise binding energy landscape of the wild type TetR transcription factor. We also generated several TetR transcription factor mutants and determined the specificities of a number of the new variants.
