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Team:UPO-Sevilla/Project/Basic Flip Flop/Modeling/Toggle Switch
From 2011.igem.org
Toggle Switch
Introduction
The next step in this point is to model the actuations that allow us to modify the state of the system, inducing a switch in the flip-flop.
These actuators are:
- Using a thermosensitive repressor protein. By increasing the temperature of the bacteria, the protein is rapidly degraded, thus helping the change.
- Adding IPTG. The IPTG molecules bind competitively to the other repressor. Therefore, the inhibition of the transcription by the repression is not permitted. The IPTG induction is achieved assuming a Hill kinetic for the ligand-binding.
Diagram
Then, the model is complemented with 3 new elements:
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