Team:Groningen/project characterisation promoters pbad

From 2011.igem.org

Revision as of 16:54, 20 September 2011 by Muzgus (Talk | contribs)


In our system and measurements we have been using the pBAD/AraC promoter ([http://partsregistry.org/Part:BBa_I0500 BBa_I0500]). This promoter is functioning in our circuit as a jammer for the LasR autoinducing loop. It is also used to induce the cI autoinducing loops during the measurements. That is why, in our opinion, characterizing this promoter in a way that would show us the activity rate not only depending on concentration of arabinose, but also in a dynamic way - over time - is crucial. The measurements were done as described in protocol.

Below you will find four figures representing the results of our measurements.

Experiment 1a. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h.
Experiment 1b. Wild type cells, induced at time=0h.
Experiment 2a. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h.
Experiment 2b. Wild type cells, induced at time=0h.




Retrieved from "http://2011.igem.org/Team:Groningen/project_characterisation_promoters_pbad"