Team:UPO-Sevilla/Project/Improving Flip Flop/Overview
From 2011.igem.org
Improving Flip Flop
This subteam, consisting of Víctor, Felix and Yolanda, tries to optimize the Basic bistable operation. The basic bistable has different weak points that we will try to solve by using a double proteolysis-asRNA mechanism.
The first problem that we will face is the tremendously long time that the bistable needs to switch from one state to the other. About this, we intend to decrease it appreciably. Furthermore, the basic bistable has a great leaky that we will also try to minimize by these mechanisms.
By implementing a proteolysis and an asRNA system, one for each part of our bistable, we will, at the same time, remove the reporter and the repressor genes, solving the problems of slowness and leaky. We will explain all this with more detail in the different sections of this wiki.