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iGEM UT-Tokyo

• LB broth

reagents	final conc.	amount
Bacto trypton	1%	1g
NaCl	0.5%	0.5g
Yeast extracs	0.5%	0.5g
MilliQ	-	100ml
Total		100ml

• SOB broth

reagents	final conc.	amount
Bacto trypton	2%	20g
NaCl	0.05%	0.5g
Yeast extracs	0.5%	5g
KCl (250mM)	2.5mM	10ml
MilliQ	-	1000ml
Total		1L

Before use, add 10ml Mg sol.

Mg sol.

MgCl2	500mM
MgSO4	500mM

• M9

• 50× TAE

reagents	final conc.	amount
Tris	2M	242g
CH3COOH	1M	57.1mL
EDTA (0.5M, pH=8.0)	0.05M	100ml
MilliQ	-	1000ml
Total		1L

• TB

reagents	final conc.	amount
KOH 500mM sol.	250mMM	242g
PIPES 500mM sol.	10mM	2ml
CaCl2 750mM sol.	align="right"15mM	2ml
KCl 2.5M sol.	250mM	10ml
MnCl2 550mM sol.	55mM	10ml
MilliQ	-	100ml
Total		100ml