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Team:Lyon-INSA-ENS/Project/Achievement
From 2011.igem.org
A race between two different strategies was used to obtain the first part
In E. coli the curli-producing system is organized in two divergeant operons with the genes csgA, csgB and csgC on one side and csgD, csgE, csgF and csgG of the other one. So to achieve the first approach of the project, it is necessary to begin by the creation of three "sub- parts": the first one would be the part with the sole promoter PrcnA, the second would be the part with the genes csgA and csgB and final one would be the part with the genes csgE, csgF and csgG.
The synthesis of this first DNA part (the cobalt-inducible promoter Prcn-csgBAEFG, which is designed to induce formation of a biofilm and to promote cell adhesion) was conceived as as a competition between two methods: a completely synthetic approach, and a "manual" method involving PCR steps followed by ligations.
