Team:Groningen/project notebook/4 July 2011

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Joyce:
PCR of HybB promotor, PcI, PlasI, LasR-LVA, cI LVA.
PCR protocol:
Denaturation: 94 °C 5 minutes
Cycle 30×
Denaturation: 94 °C 30 seconds
Annealing: 30 seconds 60 degrees
Extension: 2 minutes
Final extension: 7 minutes
Store infinite at 4 °C

Analyze on 1% agarose gel
Work on Human Practices