Team:Osaka/week8

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Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Measurment of cell survival
Table 7
NameOD600UV(mJ/cm^2)
PprI0.01090,0.5,1,2,5
PprA0.04180,0.5,1,2,5
PprM0.04540,0.5,1,2,5
RecA0.01140,0.5,1,2,5
empty vector0.05150,0.5,1,2,5
wild type0.05460,0.5,1,2,5


September 20(Tue)

September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

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