Team:Osaka/week8

From 2011.igem.org

Revision as of 02:19, 20 September 2011 by Toshi (Talk | contribs)


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Measurment of cell survival
Table 7
NameOD600UV(mJ/cm^2)
PprI①0.01090
PprI②0.01090.5
PprI③0.01091
PprI④0.01092
PprI⑤0.01095
PprA400μl2h
PprM400μl1h
RecA40μl2h
empty vector40μl1h
wild type40μl1h


September 20(Tue)

September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

Back to Notebook

Retrieved from "http://2011.igem.org/Team:Osaka/week8"