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Team:Imperial College London/Protocols Switch
From 2011.igem.org
Protocols
This page lists all the protocols used in our project. We have classified them into five main categories as follow.
Gene Guard
Genome integration
-Grow a culture of CRIM helper plasmid containing cells and make them competent.
-Transform competent cells with CRIM plasmid.
-After transformation, suspend cells in SOC without ampicillin.
-Incubate at 37°C for one hour.
-Incubate at 42°C for 30 minutes.
-Plate cells on agar plates containing kanamycin.
