Team:UT-Tokyo/Data/Method

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Reagents

LB broth

reagents	final conc.	amount
Bacto trypton	1%	1g
NaCl	0.5%	0.5g
Yeast extracs	0.5%	0.5g
MilliQ	-	100ml
Total		100ml

SOB broth

reagents	final conc.	amount
Bacto trypton	2%	20g
NaCl	0.05%	0.5g
Yeast extracs	0.5%	5g
KCl (250mM)	2.5mM	10ml
MilliQ	-	1000ml
Total		1L

Before use, add 10ml; Mg sol.

Mg sol.

MgCl2	500mM
MgSO4	500mM

M9

50× TAE
TB

Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

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