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Team:Lyon-INSA-ENS/Realisation/Collection
From 2011.igem.org
| Collection reference | Description | Resistance(s) |
|---|---|---|
| pIG1 | phaCAB | - | pIG2 | rcn-curli cloned in pUC57 plasmid | Amp |
| pIG3 | Pfort (18A: BBa_J23119) | Amp |
| pIG4 | part Bba_K342000 = Pcurli | Cm |
| pIG5 | enhanced yellow fluorescent protein derived from A. victoria GFP (24E: BBa_E0030) | Amp+K |
| pIG6 | pUC18 | Amp |
| pIG7 | pUC18 | Amp |
| pIG8 | Weak RBS (5L: Bba_J61101) | Amp |
| pIG9 | GFP with LVA tag (2L: BBa_K145015) | Amp |
| pIG10 | RBS-YFP (22M: BBa_I15017) | Amp+K |
| pIG11 | OmpR234 (BBa_K342003) | Cm |
| pIG13 | RBS-GFP-LVA (BBa_K206009) | Cm |
| pIG14 | Strong RBS (2M: BBa_B0034) | Amp |
| pIG15 | NiCoT | Amp |
| pIG16 | 18A-OmpR234 (pIG3+pIG11) | Amp |
| pIG17 | Strong RBS-YFP (pIG14+pIG10) (2M24E) | Amp |
| pIG18 | RBSfort-GFP-Tet (pIG14+pIG9) (2M2L) | T |
| pIG19 | RBSfort-GFP-Kan (pIG14+pIG9) (2M2L) | K |
| pIG20 | Prcn-csg extracted in pUC57 () | Amp |
| pIG21 | Backbone A = pSB1A3 | Amp |
| pIG22 | Backbone K = pSB1K3 | K |
| pIG23 | Backbone T = pSB1T3 | T |
| pIG24 | Backbone C = pSB1C3 | Cm |
| pIG25 | Synthetic part cloned in pSB1C3 | Cm |
| pIG26 | part Prcn (= rcnA promoter) | Cm |
| pIG27 | part Prcn (= rcnA promoter) | Cm |
| pIG28 | part Pcurli-RBS-GFP(LVA) (pIG4+pIG18) | Cm |
| pIG29 | part CsgBA | Amp |
| pIG30 | pIG25 | - |
| pIG31 | pKK-OmpR234 (p56 collection biofilm) | Amp |
| pIG32 | pPROBE OT'-Prcn-GFP (p157 collection biofilm) | Spc |
| pIG33 | PrcnA-OmpR234 | Cm |
| pIG34 | PrcnA-OmpR234 | K |
| pIG35 | = pIG25 | Cm |
| Collection reference | Description | Phenotype |
|---|---|---|
| S5 | Amp resistant | |
| S6 | NM522/pIG26 | |
| S7 | MC4100 | |
| S8 | MG1655 | |
| S9 | NM522/pIG27 | |
| S10 | NM522/pIG26 | |
| S11 | NM522 | |
| S12 | DH5α | |
| S13 | NMYO | |
| S14 | NM522 + pTNiCo | |
| S15 | MG1655 malT::Tn10 ompR234 | |
| S16 | MG1655 malT::Tn10 ompR234/ p127 (fusion PcsgBA-GFP-LVA on pPROBE) | |
| S17 | F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG25 = synthetic part rcn-curli, cloned in the backbone pBS1C3 | |
| S18 | F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG2 | |
| S19 | F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG16 | |
| S21 | F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pUC18 | |
| S22 | F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5/ pIG33 | |
| S23 | Strain derivated form MG1655 expressing constitutively GFP with pIG2 plasmid | |
| S24 | Strain derivated form MG1655 expressing constitutively GFP with pIG6 plasmid | |
| S25 | F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1/pIG6 | |
| S26 | F- [araD139]B/r Δ(argF-lac)169* &lambda- e14- flhD5301 Δ(fruK-yeiR)725 (fruA25)‡ relA1 rpsL150(strR) rbsR22 Δ(fimB-fimE)632(::IS1) deoC1/pIG2 | |
| S27 | ||
| S28 | ||
| S29 |
