From 2011.igem.org

Colony PCR with Green tag

Master mix(7ul):

1ul 10uM forward primer

1ul 10uM reverse Primer

5ul 2x Green tag

Cell water(3ul): Pick one colony from the plate and mix with 10ul of ddH2O to make 10ul of cell water

Reaction = Master mix(7ul) + Cell water(3ul) = 10ul per tube

Use program "Colony" & change the extension time (1 min per kb)