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Team:UT-Tokyo/LabNote
From 2011.igem.org
Lab Note
iGEM UT-Tokyo
Parts List
| Number | Part | Content | Plasmid | Length (bp) |
|---|---|---|---|---|
| #1 | J23119 | c.Promoter (Strong) | pSB1A2 | 35 |
| #2 | J23118 | c.Promoter (Medium) | BBa_J61002 | 35 |
| #3 | B0032 | RBS | pSB1A2 | 13 |
| #5 | B0014 | d.Ter | pSB1AK3 | 95 |
| #9 | E0240 | RBS-GFP-d.Ter | pSB1A2 | 876 |
| #10 | E0040 | GFP | pSB1A2 | 681 |
| #11 | E1010 | RFP | pSB2K3 | 723 |
| #14 | I712019 | fLuc | pSB1AK8 | 1653 |
| #17 | J52008 | rLuc | pSB1AK3 | 936 |
| #20 | R0011 | lacP | pSB1A2 | 55 |
| #21 | C0012 | LacI | pSB1A2 | 1128 |
| #22 | I712074 | pT7 | pSB1AK8 | 46 |
| #23 | K145001 | T7 RNA Pol. | pSB1A2 | 2655 |
| #24 | J22106 | recAp | pSB1A2 | 192 |
| #27 | C0083 | AspA | pSB2K3 | 1518 |
| #28 | K112808 | T4 phage lysis device (no promoter) | pSB1A2 | 1785 |
| #29 | - | CheZ | pSB1AK3 | 728 |
| #30 | K117000 | Lysis gene | pSB1A2 | 144 |
| #31 | - | LexA | pSB1AK3 | 750 |
| #33 | - | sulAp | pSB1AK3 | 67 |
| #34 | - | uvrAp | pSB1AK3 | 96 |
| #35 | - | recNp | ||
| #36 | R0051 | cI-repressed promoter | pSB1A2 | 49 |
| #37 | C0051 | cI repressor (LVA tagged) | pSB1A2 | \ |
| #38 | - | RecA | ||
| #2-9 | c.Promoter (M)-RBS-GFP-d.Ter | pSB1A2 | 911 | |
| #20-9 | lacP-RBS-GFP-d.Ter | pSB1A2 | 931 | |
| #22-9 | pT7-RBS-GFP-d.Ter | pSB1AK8 | 922 | |
| #3-11 | RBS-RFP | pSB1A2 | 736 | |
| #3-11-5 | RBS-RFP-d.Ter | pSB1AK3 | 831 | |
| pSB1C3 | 831 | |||
| #2-3-11-5 | c.Promoter(M)-RBS-RFP-d.Ter | pSB1A2 | 866 | |
| pSB1AK3 | 866 | |||
| #24-3-11-5 | recAp-RBS-RFP-d.Ter | pSB1A2 | 1023 | |
| #33-3-11-5 | sulAp-RBS-RFP-d.Ter | pSB1AK3 | 898 | |
| #14-5 | fLuc-d.Ter | pSB1AK3 | 1748 | |
| #3-14-5 | RBS-ｆLuc-d.Ter | pSB1A2 | 1761 | |
| #2-3-14-5 | c.Promoter (M)-RBS-fLuc-d.Ter | pSB1A2 | 1796 | |
| #20-3-14-5 | lacP-RBS-fLuc-d.Ter | pSB1A2 | 1816 | |
| #34-3-14-5 | uvrAp-RBS-fLuc-d.Ter | pSB1AK3 | 1857 | |
| #36-3-14-5 | cIp-RBS-fLuc-d.Ter | pSB1A2 | 1810 | |
| #17-5 | ｒLuc-d.Ter | pSB1AK3 | 1031 | |
| #3-17-5 | RBS-ｒLuc-d.Ter | pSB1A2 | 1044 | |
| #2-3-17-5 | c.Promoter (M)-RBS-ｒLuc-d.Ter | pSB1A2 | 1079 | |
| #24-3-17-5 | recAp-RBS-rLuc-d.Ter | pSB1A2 | 1236 | |
| #34-3-17-5 | uvrAp-RBS-rLuc-d.Ter | pSB1AK3 | 1140 | |
| #20-3-17-5 | lacP-RBS-ｒLuc-d.Ter | pSB1A2 | 1099 | |
| #3-14-5-2-3-17-5 | RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter | pSB1A2 | 2840 | |
| #20-3-14-5-2-3-17-5 | lacP-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter | pSB1A2 | 2895 | |
| #36-3-14-5-2-3-17-5 | cIp-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter | pSB1A2 | 2889 | |
| #3-27 | RBS-AspA | pSB1A2 | 1531 | |
| #3-27-5 | RBS-AspA-d.Ter | pSB1AK3 | 1626 | |
| #20-3-27-5 | lacP-RBS-AspA-d.Ter | pSB1A2 | 1681 | |
| #3-29 | RBS-CheZ | pSB1A2 | 741 | |
| #3-29-5 | RBS-CheZ-d.Ter | pSB1AK3 | 836 | |
| #20-3-29-5 | lacP-RBS-CheZ-d.Ter | pSB1A2 | 891 | |
| #36-3-29-5 | cIp-RBS-CheZ-d.Ter | pSB1A2 | 885 | |
| #37-5 | cI-d.Ter | pSB1AK3 | #VALUE! | |
| #3-37-5 | RBS-cI-d.Ter | pSB1A2 | #VALUE! | |
| #20-3-37-5 | lacP-RBS-cI-d.Ter | pSB1A2 | #VALUE! | |
| #20-28 | lacP-〈T4 phage lysis device〉 | pSB1A2 | 1840 | |
| #3-30 | RBS-Lysis gene | pSB1A2 | 157 | |
| #3-23 | RBS-T7 RNA Pol. | pSB1A2 | 2668 | |
| pSB1A2 | Plasmid backbone | pSB1A2 | 2079 | |
| pSB1AK3 | Plasmid backbone | pSB1AK3 | 3189 | |
| pSB1A3 | Linearized plasmid backbone | pSB1A3 | 2155 | |
| pSB1K3 | Linearized plasmid backbone | pSB1K3 | 2204 | |
| pSB1C3 | Linearized plasmid backbone | pSB1C3 | 2070 | |
| pSB1C3 | J04450 | lacP-RBS-RFP-d.Ter | pSB1C3 | 1069 |
Lab Diary
For convenience sake, each part (gene, promotors, etc) are represented by consecutive numbers (e.g. #20 for lac promotor). By pointing the mouse on the part number in the labnote, you can find out the details of the part.
