Experiments of the YFP concentration design

The planning of the experiments is the following : first we have tested the strains from D. Lane containing YFP:tetR and tetO array. Then we constructed/biobricked the YFP:tetR and tetO array system. To finish with the microscopy step and results of this proof of concept between B. subtilis and B. subtilis / E. coli.

Testing the YFP:tetR strains from D. Lane

In the article [1], E. coli strains are growing at 20°C to avoid protein agregation but the problem is that nanotube between B. subtilis has been only proved to exist at 37°C. We test different possibilities : at 37°C or 30°C and concentration of arabinose (0% - 0,1% -0,2%) to deal with protein agregation.

tetR:YFP / tetO array : 37°C
tetR:YFP / tetO array inducted with no arabinose on E. Coli from Dave Lane plasmids.	tetR:YFP / tetO array inducted with no arabinose on E. Coli from Dave Lane plasmids.
tetR:YFP / tetO array inducted with 0,2% arabinose on E. Coli from Dave Lane plasmids.	tetR:YFP / tetO array inducted with 0,2% arabinose on E. Coli from Dave Lane plasmids.

tetR:YFP / tetO array : 30°C
tetR:YFP / tetO array inducted with no arabinose on E. Coli from Dave Lane plasmids.	tetR:YFP / tetO array inducted with no arabinose on E. Coli from Dave Lane plasmids.
tetR:YFP / tetO array inducted with 0,2% arabinose on E. Coli from Dave Lane plasmids.	tetR:YFP / tetO array inducted with 0,2% arabinose on E. Coli from Dave Lane plasmids.