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Team:Washington/Alkanes/Methods
From 2011.igem.org
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In order to produce alkanes, we need both Acyl-ACP Reductase and Aldehyde Decarbonylase to work together in the cells, which is the basic system.
Contents |
Building a Perfect Alkane Production System
Our goal is not only to build a system that produces alkane with Acyl-ACP Reductase and Aldehyde Decarbonylase, but also to modify the whole system on producing shorter length of alkanes, higher yield of production of alkanes, higher quality of alkanes (branched alkanes), etc. In order to achieve all these, we have groups work on six different directions: system optimization, alternative chassis, decarbonylase redesign, alternative aldehyde, enzyme localization, branched alkanes.
System Optimization
Single plasmid vs. double plasmid.
alternative chassis
The idea of alternative chassis is to run the basic system on different types vectors.
