Team:Tsinghua/Notebook/9 September 2011

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pSB1C3-Em7-OHS 

 digest OHS with EcoRI, NEB, for 1 h, then XbaI, NEB, for 2h
 heat inactive
 anneal Em7 ssoligo
 ligate

pSB1C3-Tar 

 mini pre. No.1 2 4

pSB1C3-PAA 

 mini pre. PAA2
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