Team:Tsinghua/Notebook/9 September 2011

From 2011.igem.org

pSB1C3-Em7-OHS

 digest OHS with EcoRI, NEB, for 1 h, then XbaI, NEB, for 2h
 heat inactive
 anneal Em7 ssoligo
 ligate

pSB1C3-Tar

 mini pre. No.1 2 4

pSB1C3-PAA

 mini pre. PAA2