Team:Paris Liliane Bettencourt/Notebook/2011/09/08/

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Team IGEM Paris 2011

Contents

Cyrille

Digestion and gel

S24 is prepared to be cloned with

  • RFP - TT
  • T7 amber - TT
  • ComS
  • KinA-TT

3*4 500ng of S24 was digested and runned on the gel The insert was digested several time.

Then the gel was runned and the bands cutted.

S24 digested - S24a 1,2,3,4 - S24 b 1, 2,3, b - S24c 1,2,3,4
S24 bands cutted
RFP Ter 1,2,3 - T7 amber ter 1,2,3 - KinA-ter 1,2,3
RFP ter cutted
T7 ter cutted
KinA ter cutted

Then I proceed to a gel extraction of the bands.

PCR colony of TetO/TetR

New attempt of PCR colony to find one good clone. Runned on a gel

PCR colony 2,4,5,6,7,8,9,10

Miniprep of YFP-TetR BB

Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red)

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