Team:Paris Liliane Bettencourt/Notebook/2011/09/05/

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Team IGEM Paris 2011

Contents

Cyrille

Miniprep

Miniprep of 2 clones of TetO array in pSB1C3, 2 clones of TetO array in pHM3 Miniprep of Mathias: S21 S82 1 2 3 4; S75 S67 and S75

With good yeilds (>500ng)

Gel Extraction

Gel extraction of pHM3 Digested in EP and pVeg YFP TetR D EP and DXP.

Ligation

Ligation of pHM3 Digested EP with pVeg-YFP-TetR D. EP with ratio 1:1 1:10 1:100 Ligation pHM3 TetO D. SP with pVeg-YFP-TetR D. SP

Transformation

Transformation of the ligation product + ComS ligated in pSB1C3 + YFP TetR BB ligated in pSB1C3

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