Team:Tsinghua/Notebook/4 July 2011

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Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug)

Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction.


Ligation

1ul vector + 10ul GFP + 10ul Takara Solution I

RT, 1h

Transform DH5a competent cells, spread on Amp+ LB plates.

YX

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