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From 2011.igem.org

pET15b OmpA-GFP construct

 Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug)
 Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction.
 Ligation
 1ul vector + 10ul GFP + 10ul Takara Solution I. RT, 1h
 Transform DH5a competent cells, spread on Amp+ LB plates.
 YX

pet15b OmpA-HIV cleavege site-sh3

 PCR OmpA-HIV site&HIV site-sh3
 overlapping pcr
 gel extraction