Team:Bielefeld-Germany/HPLC isocratic

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HPLC method

  • C18 reverse phase column
  • Isocratic method: 45% acetonitril
  • Flow = 0.6 mL/min
  • UV-detection at 227 nm
  • Column:
    • Eurospher II 100-5 C18p
    • Dimensions: 150 x 4.6 mm with precolumn
    • Particle size: 5 µm
    • Pore size: 100 Å
    • Material: silica gel
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