Team:Bielefeld-Germany/HPLC isocratic

From 2011.igem.org

HPLC method

  • C18 reverse phase column
  • Isocratic method: 45 % Acetonitrile
  • Flow = 0.6 mL min-1
  • UV-detection at 227 nm
  • Internal standard: 100 mg L-1 Bisphenol F (BPF)
  • Column:
    • Eurospher II 100-5 C18p by Knauer
    • Dimensions: 150 x 4.6 mm with precolumn
    • Particle size: 5 µm
    • Pore size: 100 Å
    • Material: silica gel
  • Software:
  • Autosampler:
  • Pump:
    • L-6200A Intelligent Pump by Hitachi
  • UV-Detector:
Chromatogram obtained using the above described materials and methods. Three distinctive peaks are of interest: BPA at 7,3 min retention time, BPF at 5,8 min retention time and the degradation product of BPA at 4,6 min retention time.