green light receptor
Quickchange
Investigator:Jakob
PCR
| Name:
Jakob
| Date:
08.08.2011
|
| Continue from Experiment:
|
| Project Name:
Quickchange CcaR and CcaS
|
PCR-Mixture for one Reaction:
For a 50 µl reaction use
| 32,5µl
| H20
|
|
| 10µl
| 5x Phusion Buffer
|
|
| 2.5µl
| Primer fw
| Primer: P12(CcaR) and P14(CcaS
|
| 2.5µl
| Primer dw
| Primer: P13(CcaR) and P15(CcaS)
|
| 1µl
| dNTPs
|
|
| 1µl
| DNA-Template
| DNA: CcaR and CcaS
|
| 0.5 µl
| Phusion (add in the end)
|
|
Digest
with DpnI
Transformation
blue light receptor
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red light receptor
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Lysis cassette
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Precipitator
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