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Team:EPF-Lausanne/Protocols/Agar Plates
From 2011.igem.org
Agar plate preparation
Back to protocols.Agar plates are used for cell cultures. Their preparation is relatively simple: first an agar broth is mixed and autoclaved. Then an antibacterial agent such as Ampicillin is mixed in. Finally, the solution is poured into disposable petri plates and let gelify for 20 minutes around a bunzen burner flame, as illustrated below.
Agar broth
| Product | Amount |
|---|---|
| NaCl | 10 g |
| Tryptone | 10 g |
| Yeast Extract | 5 g |
| Agar | 20 g |
| H2O (deionised) | fill to 1 l |
Pouring the plates
Arrange empty petri dishes in a crescent around a Bunzen burner. Half-fill them with agar broth. Stack another layer of empty dishes above, and repeat until all the broth has been poured. One liter of broth fills approximately 30 plates.
Leave the plates around the flame for approximately twenty minutes, until the gel is solidified.
