Team:Cornell/Week 6

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Revision as of 03:47, 18 July 2011 by Bwj8 (Talk | contribs)

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June 10th - June 16th

Sunday

  • Worked on Website. Preliminary design of banner, safety content, and temporary lab notebook spreadsheet.
  • Ligation product was transformed in electrocompetent cells, culture plated on Amp
  • Vio-Operon plasmid was transformed and plated on Kanamyacin
  • RFP PCR done overnight

Monday

Tuesday

Wednesday

Thursday

  • Sequencing revealed no GFP. Retry the 3-piece ligation of GFP + Annealed Primer Pair A (half of biotin tag) + Annealed Primer Pair B (other half of biotin tag & iGEM suffix).
  • PCR to amplify vector backbone containing RFP.
  • Met Prof. Lucks -- prospective iGEM adviser and coolest man alive

Friday

Team Meeting

Saturday